PNAs are expected to last a lot longer than shRNA because they can't be degraded by any known nucleases. I would guess that once the PNA enters a cell, it'll be stuck there for long time.
Thanks for the great comment!
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Thank you for the great article!
This is very interesting, because that would mean that you could potentially have "permanent" knockdown of a gene, something like with CRISPR, but better, because gene itself would retain its function.
At the moment, I'm struggling with the analyses of shRNA and CRISPR Achilles data sets, and when you compare dependency profiles for the same gene, the results are quite different, which is of course expected, since we're dealing with complete different methods (gene knockdown vs. gene knockout).
I would be very happy to see in the future some Achilles PNAs dependencies project :)
Definitely. It's possible that PNA might render some of the other silencing/knockout methods obsolete if it becomes easy to synthesize.