exTEN 2X PCR Master Mix - A Master Mix For Polymerase Chain Reactions Applications

in #steemhunt7 years ago

exTEN 2X PCR Master Mix

A Master Mix For Polymerase Chain Reactions Applications


Screenshots

exten 1.png


Hunter's comment

The exTEN 2X PCR Master Mix is a special premixed polymerase chain reaction blend which is made up of dNTPs, Magnesium chloride, Taq DNA Polymerase, a proofreading enzyme and reaction buffer for the creation of multiple copies of DNA templates. Since a 3' 5' exonuclease (proofreading) ability is added, it improves the efficiency in the creation of multiple copies of DNA templates with the use of a lower error rate of misincorporated nucleotides when compared to Taq DNA polymerase alone.

The exTEN 2X PCR Master Mix yields better results and reproduces longer DNA fragments. By reducing the amount of pipetting steps required for setting up a polymerase chain reaction, the exTEN 2X PCR Master Mix is of immense help in the task of reducing the risk of contamination as well as help to save time.

This mix is a welcome change in the world of Biochemistry.


Some Applications Of The exTEN 2XPCR Master Mix

  • Labelling Of DNA
  • Sequencing Of DNA
  • Polymerase Chain Reaction amplification of fragments of DNA
  • Polymerase Chain Reaction For Cloning

Merits Of The exTEN 2XPCR Master Mix

  • It increases the success rates of the polymerase chain reaction with improved yields and greater replication of DNA fragments up to 10kb

  • Because it is designed for direct loading and easy preparation, the risk of errors and contamination is reduced as little pipetting steps are required.

  • Even when the concentration of the DNA template is low, better sensitivity is achieved.

  • Reduced preparation time.

  • It is convenient and easy to handle


Link

http://www.base-asia.com


Contributors

Hunter: @illuminatus



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Pros:

  • This is a very amazing idea
  • It can make a change in the world of biochemistry

Cons:

  • None

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