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If it has 16 fans now it will only ever have 16 fans until you empty it. Then when it's empty it will be filled again with more temporary fans. You can choose to empty it at any time but it's best to do so (if you're wanting to increase your fans) when you are going to be playing for a decent amount of time as the fans last for 12 hours.

If you ego is high it's best to leave them in the bottle until you'be bought your ego down.

So I use the bottle in the Morning If I Should play a lot a day. Will try that. Thanks