What I do, I repeat the presentation in front of a mirror, imagining double the people in attendance than what is expected, so when you see them your brain will be like Meh, not even half of them, so you will relax after that. It works.
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I remember at Uni being told to repeat the lecture in the mirror, as it helps teach yourself as the lecturer.
This isn't one of those presentations though unfortunately, it is quite dynamic and requires interaction from the audience. I just have to know the content well enough and then rely on engaging them. It should be okay, but I haven't delivered to such a large group in a very long time.
I am sure you will do alright. Like the bike, we never forget how to do it.